Plasmid
Part:BBa_K076015
Designed by: iGEM2008 Princeton team Group: iGEM08_Princeton (2008-10-29)
pLV-TRE-Mash1-IRES2-mKate
This plasmid encourages differentiation of embryonic stem cells into neuronal precursors and marks the cells with the red-fluorescent mKate.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1488
Illegal XbaI site found at 8889
Illegal SpeI site found at 8166
Illegal SpeI site found at 8840
Illegal PstI site found at 972
Illegal PstI site found at 3466
Illegal PstI site found at 10018 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1488
Illegal NheI site found at 1507
Illegal SpeI site found at 8166
Illegal SpeI site found at 8840
Illegal PstI site found at 972
Illegal PstI site found at 3466
Illegal PstI site found at 10018
Illegal NotI site found at 85
Illegal NotI site found at 2865
Illegal NotI site found at 8876
Illegal NotI site found at 9640 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1488
Illegal BglII site found at 3630
Illegal BglII site found at 3696
Illegal BglII site found at 3737
Illegal BglII site found at 7929
Illegal BglII site found at 8934
Illegal BamHI site found at 1546
Illegal BamHI site found at 10159
Illegal XhoI site found at 8883 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1488
Illegal XbaI site found at 8889
Illegal SpeI site found at 8166
Illegal SpeI site found at 8840
Illegal PstI site found at 972
Illegal PstI site found at 3466
Illegal PstI site found at 10018 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1488
Illegal XbaI site found at 8889
Illegal SpeI site found at 8166
Illegal SpeI site found at 8840
Illegal PstI site found at 972
Illegal PstI site found at 3466
Illegal PstI site found at 10018
Illegal NgoMIV site found at 4325
Illegal NgoMIV site found at 5358
Illegal NgoMIV site found at 5419
Illegal NgoMIV site found at 5533
Illegal NgoMIV site found at 9556
Illegal NgoMIV site found at 9812
Illegal NgoMIV site found at 9858 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3719
Illegal BsaI site found at 8916
Illegal BsaI site found at 10134
Illegal BsaI.rc site found at 3479
Illegal BsaI.rc site found at 7061
Illegal BsaI.rc site found at 8799
Illegal BsaI.rc site found at 10861
Illegal SapI site found at 5978
Illegal SapI.rc site found at 49
Illegal SapI.rc site found at 10243
[edit]
Categories
Parameters
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