Plasmid

Part:BBa_K076015

Designed by: iGEM2008 Princeton team   Group: iGEM08_Princeton   (2008-10-29)


pLV-TRE-Mash1-IRES2-mKate

This plasmid encourages differentiation of embryonic stem cells into neuronal precursors and marks the cells with the red-fluorescent mKate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1488
    Illegal XbaI site found at 8889
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal PstI site found at 10018
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1488
    Illegal NheI site found at 1507
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal PstI site found at 10018
    Illegal NotI site found at 85
    Illegal NotI site found at 2865
    Illegal NotI site found at 8876
    Illegal NotI site found at 9640
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1488
    Illegal BglII site found at 3630
    Illegal BglII site found at 3696
    Illegal BglII site found at 3737
    Illegal BglII site found at 7929
    Illegal BglII site found at 8934
    Illegal BamHI site found at 1546
    Illegal BamHI site found at 10159
    Illegal XhoI site found at 8883
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1488
    Illegal XbaI site found at 8889
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal PstI site found at 10018
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1488
    Illegal XbaI site found at 8889
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal PstI site found at 10018
    Illegal NgoMIV site found at 4325
    Illegal NgoMIV site found at 5358
    Illegal NgoMIV site found at 5419
    Illegal NgoMIV site found at 5533
    Illegal NgoMIV site found at 9556
    Illegal NgoMIV site found at 9812
    Illegal NgoMIV site found at 9858
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3719
    Illegal BsaI site found at 8916
    Illegal BsaI site found at 10134
    Illegal BsaI.rc site found at 3479
    Illegal BsaI.rc site found at 7061
    Illegal BsaI.rc site found at 8799
    Illegal BsaI.rc site found at 10861
    Illegal SapI site found at 5978
    Illegal SapI.rc site found at 49
    Illegal SapI.rc site found at 10243


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